|
KMID : 0364819870250030189
|
|
Korean Journal of Microbiology
1987 Volume.25 No. 3 p.189 ~ p.197
|
|
|
Regulation of Cell Growth and Tylosin Biosynthesis through Flux Control of Metabolic Intermediate in Streptomyces fradiae
|
|
Kang Hyun-Ah
Lee Kye-Joon
|
|
|
Abstract
|
|
|
1
The aim of the present study was to investigate the effect of glutamate on the biosynthesis of tylosin. Activities of enzymes involved in the metabolic pathway of glutamate to form tylactone, an essential precursor of tylosia, were determined using Streptomyces fradiae grown at different concentration of glutamate. As results, it was found that cell growth and tylactone formation was controlled by the metabolic flux of oxaloacetate. It was dear that cell growth was favored by the activities of citrate synthase and aspartate aminotransferase, while the tylactone synthesis was stimulated by the activity of methylmalonyl-CoA carboxyltransferase. Therefore it was concluded that channelling of oxaloacetate was a point for favoring either cell growth or tylosin biosynthesis.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|